Biotin Protein (Alkaline Phosphatase (AP))

N° du produit ABIN964125

N° du produit (Fournisseur): b000-05

Cette protéine Biotin est exprimée dans Synthetic.

Aperçu rapide pour Biotin Protein (Alkaline Phosphatase (AP)) (ABIN964125)

Antigène: Biotin

Origine: Chemical

Source: Synthetic

Application: Dot Blot (DB), ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Détail du produit

Purification/Conjugué: Cette Biotin protéine est marqué à la Alkaline Phosphatase (AP).

N° du produit (Fournisseur): b000-05

Fournisseur: Rockland

Fonction: Biotin Alkaline Phosphatase Conjugated

Purification: This product was prepared from electrophoretically pure Alkaline Phosphatase and Biotin.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Biotin.

Stérilité: Sterile filtered

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: Biotin Alkaline Phosphatase Conjugated has been tested by ELISA and dot blot and can be utilized in ELISA and Western Blotting experiments where the assay's target of interest is coupled with streptavidin.

Western Blot Dilution: 1:500 - 1:2,500

ELISA Dilution: 1:8,000 - 1:32,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of receipt.

Date de péremption: 12 months

Détail du antigène

Antigène: Biotin

Classe de substances: Chemical

Sujet:

Synonyms: Biotin Alkaline Phosphatase Conjugated, Biotin alk phos Conjugated, alkaline phosphatase conjugated biotin

Background: Biotin is a small biomolecule important for many cellular processes. Most importantly for biotechnology applications, biotin is ammenable to conjugation to proteins for use in biochemical assays. Biotin has a very strong affinity for avidin and streptav